Molecular Biology & Biochemistry Consulting
Doctor
Yogeshwari Singh
Expert consulting in molecular biology, protein biochemistry, RNA biology, and scientific communication.
Partner with Dr. Yogeshwari Singh for research support and technical writing.
About Me
I am Dr. Yogeshwari Singh, a scientific researcher and consultant with a Ph.D. in Biological Sciences, an MBA, and 8+ years of research experience. My work spans molecular biology, protein biochemistry, RNA biology, structural biology, and biophysical characterization, with experience designing and executing independent research projects and developing and optimizing biochemical and molecular assays.
My technical expertise includes protein and RNA characterization, protein purification, molecular biology, RNA analysis, Northern blotting, enzyme kinetics, FPLC/SEC, SDS-PAGE, surface plasmon resonance (SPR), bio-layer interferometry (BLI), microscopy, structural biology, X-ray crystallography, and protein–protein and protein–ligand interaction assays. I also have experience analyzing complex scientific data, reviewing scientific literature, troubleshooting experimental challenges, and developing reproducible research workflows.
Alongside my research expertise, I specialize in scientific and technical communication. I develop scientific manuscripts, research summaries, technical materials, presentations, posters, and other research content for diverse audiences. My combined background in biological sciences and business also enables me to approach research from both technical and strategic perspectives, supporting project planning, and the clear translation of complex scientific concepts.
Education background
Ph.D. in Biological Sciences
(01/2021 – 08/2026)
My doctoral-level training in Biological Sciences provides the scientific foundation for my work across molecular biology, protein biochemistry, RNA biology, structural biology, biochemical assays, and biophysical characterization, along with experience as a teaching assistant.
Master of Business Administration (MBA)
(07/2022 – 07/2025 )
My MBA complements my scientific background with a business and strategic perspective, supporting my approach to project planning, cross-functional communication, and understanding the broader context of research.
Master’s in Microbiology
(07/2018 – 07/2020)
My master’s training in Microbiology provided a strong foundation in microbiological research, molecular biology, and experimental methods. My research focused on Mycobacterium smegmatis as a model for studying tuberculosis-related biology, providing hands-on experience in microbial culture, molecular techniques, experimental design, and scientific analysis.
Bachelor’s in Biology
(07/2015 – 07/2018)
Research
My Expertise
Molecular & RNA Biology
I work across molecular biology and RNA biology, with experience in molecular cloning, PCR, primer design, plasmid construction, targeted mutagenesis, in vitro transcription (IVT), radiolabeled RNA, RNA characterization, Northern blotting, and biochemical RNA assays.
Protein Biochemistry & Characterization
My experience includes recombinant protein expression and purification, FPLC/SEC, SDS-PAGE, enzyme kinetics, biochemical assay development and optimization, protein characterization, and analysis of protein–protein and protein–ligand interactions.
Biophysical, Structural & Cell Biology
I have experience using SPR, BLI, and MST for biophysical characterization, along with X-ray crystallography and structural biology to investigate molecular interactions and mechanisms. My cellular research experience includes cell culture, iPSC-based mono- and co-culture systems, cancer patient-derived cells, and fluorescence microscopy to study protein localization and cellular processes.
Scientific Communication & Business Perspective
My experience includes scientific presentations, technical writing, research publications, teaching, mentoring, scientific outreach, and communicating complex research to diverse audiences. My MBA complements my scientific training with a business and strategic perspective, supporting project planning, cross-functional communication, and understanding the broader context of research.
Projects
NAD-RNA Decapping & RNA Biology
My doctoral research focused on understanding NAD-RNA decapping and its regulation by the E. coli enzymes NudC and RppH and the signaling molecule ppGpp. I investigated how these enzymes recognize and process NAD⁺-capped RNA and how ppGpp influences their decapping activity.
My work combined molecular biology, RNA biochemistry, and biophysical approaches, including in vitro transcription, radiolabeled RNA substrates, RNA decapping assays, Northern blotting, SPR, BLI, and enzyme kinetics. I also used X-ray crystallography and structural analysis to investigate protein–ligand interactions and the molecular basis of NudC function.
This research resulted in a published method for measuring enzymatic RNA decapping activity and contributed to additional manuscripts in preparation on ppGpp-regulated NAD-RNA decapping and the cellular impact of NudC and RppH through integrated transcriptomic and proteomic analysis.
Protein, Biophysical & Cell Biology
My research experience spans protein expression, purification, characterization, and biomolecular interaction analysis. I have worked with FPLC/SEC, SDS-PAGE, enzyme kinetics, biochemical assay development and optimization, and protein–protein and protein–ligand interaction assays, using SPR, BLI, and MST for quantitative biophysical characterization.
I have also applied structural biology approaches, including X-ray crystallography, to investigate protein structure and molecular interactions. My work contributed to research on the E. coli DEAD-box ATPase CsdA as an NAD⁺-capped RNA binding protein and its interaction with the NAD-RNA decapping enzyme NudC.
Beyond purified protein systems, my experience includes cell culture and translational research at Nemours, working with iPSC-based mono- and co-culture systems and cancer patient-derived cells. I have also used fluorescence microscopy to investigate protein localization and cellular processes, providing complementary cellular and molecular perspectives.
Teaching, Mentoring, and Outreach
My professional experience extends beyond laboratory research to teaching, mentoring, scientific presentations, networking, outreach, and community engagement. As a teaching assistant, I supported undergraduate learning in biological sciences and laboratory-based courses, and I have also mentored students and researchers and delivered guest lectures and scientific presentations.
My MBA complements my scientific training with a business and strategic perspective, supporting my approach to project planning, cross-functional communication, relationship building, and understanding the broader context of research. I have also participated in scientific conferences, professional networking, and events that have allowed me to connect with researchers and professionals across different areas of science.
I have contributed to scientific outreach and volunteering activities, including speaking to students through Women in STEM initiatives and engaging broader audiences with scientific concepts and research. These experiences have strengthened my ability to communicate complex scientific information clearly and build meaningful connections across academic, professional, and community settings.
Professional Development / MBA
MBA & Business Perspective
My MBA complements my scientific training with a business and strategic perspective, supporting my approach to project planning, operations, communication, and understanding the broader context of scientific research. My business coursework has included Managing People & Organizations, Operations Management, Communicating, and Professional Branding & Career Networking.
Professional Skills
My professional experience extends beyond technical research to include cross-functional collaboration, project coordination, stakeholder communication, relationship building, technical communication, scientific storytelling, problem solving, decision making, and professional networking. These skills allow me to connect scientific expertise with broader research, organizational, and business goals.
Conferences, Networking & Career Development
I have actively participated in scientific conferences, professional networking events, workshops, and STEM outreach activities. These experiences have provided opportunities to connect with researchers and professionals across different fields, expand my professional network, share scientific knowledge, and continue developing as a scientist and science professional.
Publications
Published papers
A gel electrophoresis-based assay for measuring enzymatic RNA decapping activity
Developed and described a gel electrophoresis-based assay for measuring enzymatic RNA decapping activity, providing a straightforward approach to evaluate RNA decapping reactions and enzyme activity. This work established a reproducible method for studying RNA 5′-end processing and decapping.
The E. coli DEAD Box ATPase CsdA Is a NAD+ Capped RNA Binding Protein
Contributed to research demonstrating that the E. coli DEAD-box ATPase CsdA binds NAD⁺-capped RNA, providing insights into the recognition and regulation of NAD⁺-capped transcripts in bacterial RNA biology.
ppGpp Regulates NAD-RNA Decapping by E. coli NudC and RppH
The regulation of NAD-RNA decapping by the E. coli enzymes NudC and RppH. This work investigates how ppGpp modulates NAD-RNA decapping activity and provides insight into the connection between RNA processing and bacterial stress signaling. (Manuscript in preparation)
Integrated Transcriptomic and Proteomic Analysis Reveals the Cellular Impact of NAD-RNA Decapping by NudC and RppH
An integrated transcriptomic and proteomic analysis of the cellular impact of NAD-RNA decapping by NudC and RppH. This work combines RNA-seq and quantitative proteomics to investigate how loss of NudC and RppH affects bacterial gene expression and cellular pathways.(Manuscript in preparation)
Leadership, Management & Technical Communication
Laboratory Safety & Management
I have supported laboratory safety, compliance, and day-to-day research operations, including serving as a BSL-2 Safety Manager and participating in departmental laboratory safety activities. These responsibilities included promoting safe laboratory practices, supporting safety procedures, and helping maintain a safe and compliant research environment.
Scientific & Technical Writing
My scientific writing experience includes research manuscripts, technical documentation, research summaries, grant-related writing, and scientific communication for diverse audiences. I have contributed to research proposals and funding applications and have experience translating complex scientific information into clear, structured, and accessible written content.
Teaching & Training
As a Teaching Assistant at the University of Delaware, I trained more than 100 students in laboratory techniques, accurate experimental execution, and laboratory documentation. I also mentored undergraduate researchers, helping them develop independent experimental skills and confidence in laboratory-based research. I have additionally served as a guest lecturer for introductory microbiology and shared scientific knowledge through academic and STEM-focused presentations.
Scientific Leadership & Professional Service
My academic and professional involvement includes organizing activities within the Department Microbiology Group (G3), serving on the Executive Board of the Biological Sciences Graduate Student Association (BGSA), and participating in scientific and professional organizations. I have also contributed to STEM outreach through speaking engagements with the University of Delaware Women in STEM Club and remain engaged with the broader microbiology community.
Research & Professional Development
Clinical & Diagnostic Laboratory Experience
As a Molecular Biology Technician at a State Virology Laboratory, I performed RT-PCR testing on clinical samples during COVID-19 diagnostic operations. This experience involved processing biological samples, following established testing procedures, and maintaining traceable testing documentation in a high-throughput laboratory environment.
My professional development has included competitive research and professional development awards, including the Delaware INBRE Core Facility Award for RNA Sequencing, the University of Delaware Graduate Student Travel Award, and the Departmental Professional Development Award. I am also a Certified CyberAmbassador through an NSF-supported program, reflecting additional training in cybersecurity awareness and responsible research practices.
Talks & Presentations
Conference Presentations
- Microbiology Symposium — Research presentation on NAD-RNA metabolism and RNA decapping
- InnovateHER Conference — Scientific research presentation
- Other scientific and professional conferences — Research presentations and discussions related to molecular biology, RNA biology, and protein biochemistry
Scientific Seminars
- Biological Research Department Seminar, University of Delaware — Research presentation on NAD-RNA decapping, NudC, RppH, and ppGpp
- Departmental Research Presentations — Presentations of doctoral research and ongoing work in RNA biology and molecular mechanisms
Research Posters
- Gordon Research Conference (GRC) — Research poster presentation
- Transcription Conference, Virginia — Research poster presentation
- Additional Scientific Meetings — Research posters covering RNA biology, NAD-RNA decapping, protein characterization, and related research
Research Interviews
- Ph.D. Research Interviews — Discussions of doctoral research, experimental approaches, scientific expertise, and research findings
- Industry Research Interviews — Technical presentations and discussions covering molecular biology, protein characterization, biochemical assays, structural biology, and related research experience
Professional Research Presentations
- Women in STEM Club, University of Delaware — Invited speaker discussing scientific research and experiences in STEM
- Guest Lecturer, Introductory Microbiology (BISC 300), University of Delaware — Lecture on microbiology and related biological concepts
- Scientific Research Presentations — Presentations communicating research findings and technical concepts to academic and professional audiences
Testimonials
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My resume
protein biochemistry, RNA biology, and scientific communication.